The HIPPEX Centrifugation Medium (HCM) is delivered as a powder in a plastic bottle, hermetically sealed in order to make 50 ml HCM

50 ml demineralized water (37°C) needs to be added in the bottle. After gentle shaking and when the powder is completely dissolved the HCM ready to use.

The ejaculate that arrives in the lab should be diluted with the HCM in a 1:1 ratio and then centrifuged.

After centrifugation and removal of the supernatant, the concentrated sperm is returned to the original ejaculatory volume with HIPPEX (D2) for fresh or cooled semen